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Dls malvern zetasizer nano series software full#
Dls malvern zetasizer nano series software software#

Pre-screening: Ideal to check sample quality before running other expensive and time-consuming biophysical methods such as TEM, AUC, SAXS/SANS, FFF and SEC.įor in-depth characterization of high-resolution size distributions and other physical properties, SEC-MALS and FFF-MALS offer complementary and orthogonal techniques to DLS.ĭLS determines the hydrodynamic size of a nanoparticle in solution by measuring the time scale of Brownian motion.Advanced properties: Measure particle concentration, nonspecific interactions ( A 2 or B 22) and differentiate between conformational changes and aggregation with the static light scattering option in the NanoStar and Plate Reader.Versatility: Beyond size and size distributions, measure other properties such as stability, stress-induced aggregation and molecular interactions.No shearing or filtering: always measure what’s in your solution.Low sample volume: Only 2 µL in the DynaPro NanoStar using quartz cuvettes, 4 µL in disposable cuvettes or standard microwell plates in the DynaPro Plate Reader.Non-destructive and fully recoverable: Samples may be transferred to other assays.

Dls malvern zetasizer nano series software full#

Full nanometer size range: from 0.5 nm to 2.5 µm in a single measurement, with a resolution up to a factor of 3-5 in size.

Dynamic & Electrophoretic Light ScatteringĭLS is an ideal screening tool for biopharmaceutical formulations and other nanomaterials.

The radius at pH 7 is 2.7 nm, which is consistent with an estimated molecular weight of 34.1 kDa – nearly identical to the known value.įigure 2: Size distributions for human and bovine insulin at pH 2 and 7, measured with a Malvern Instruments Zetasizer Nano system.įor additional questions or information regarding Malvern Instruments complete line of particle and materials characterization products, visit us at. At pH 7, insulin has a hexameric quaternary structure with a known molecular w eight of 34.2 kDa. The hydrodynamic radius at pH 2 is 1.7 nm, which is consistent with a molecular weight of 12.1 kDa using the globular protein calibration curve. At pH 2, insulin has a dimeric structure with known molecular weight of 11.4 kDa. The DLS measured size distributions for human and bovine insulin at pH 2 and 7 are shown in Figure 2. For reference purposes, it is noted that all of the proteins in the protein family are globular the linear polymers are Pullulans or linear polysaccharides the branched polymers are Ficolls or densely branched polysaccharides and the spherical polymers are starburst type dendrimers, described as spherical, with a density that increases with radial distance from the core.įigure 1: Molecular family dependent empirical mass vs size calibration curves for use in DLS applications for estimating molecular weight from measured hydrodynamic size.Ī nice example for illustrating the value of DLS based molecular weight estimates is the evaluation of the quaternary structure of insulin.

Dls malvern zetasizer nano series software software#

The calibration curves used for estimating molecular weight from DLS data in the DTS software for the Malvern Zetasizer Nano system are shown in Figure 1. However, empirical mass vs size calibration curves, each particular to a specific ‘family’ or type of macromolecule, are available for use in DLS applications – assuming of course, that some a priori information regarding molecular conformation is known. With GPC, a molecular weight vs elution time or migration distance calibration curve is used with DLS, a molecular weight vs hydrodynamic size calibration curve is used.Īs with GPC techniques, the correlation between the specific volume (1/ r ) of a particle and its tertiary conformation complicates the development of a universal calibration curve for DLS. With regard to estimating the molecular weight from a dynamic light scattering measurement, an approach similar to that used in the GPC technique can be applied. GPC, HPLC, and dynamic light scattering will only give molecular weight estimates, by comparison of a measured property to the same property measured for a series of standards of assumed similarity.

Under the category of "absolute molecular weight", ultra-centrifugation, MS, and static light scattering are the only techniques listed above that will give one an absolute measurement, i.e. There are a variety of methods available to researchers for measuring or estimating the molecular weight of macromolecules, including gel permeation chromatography (GPC), liquid chromatography (HPLC), ultra-centrifugation, mass spectroscopy (MS), static light scattering (SLS), and dynamic light scattering (DLS). Can_the_molecular_weight_be_measured_with_DLS_ Can the molecular weight be measured with DLS?